Sodium polyanethol sulphonate discs to identify Gardnerella vaginalis.

Sodium polyanetholesulfonate in the identification of Gardnerella vaginalis.

With respect to paragraph 6 of Dr. L0vborg's letter, the solid phase was blocked with BSA and Tween as described. The assessment of the plates did not occur in only a few experiments. We worked with the Nunc plates using the complete ELISA procedure as described for many months before evaluating other types of plates. Finally, the view held by Dr. L0vborg in paragraph 3 that detergent is a weak...

Biotypes of Gardnerella vaginalis.

A simple and reproducible scheme for identifying biotypes of Gardnerella vaginalis has been developed, based on reactions for lipase, hippurate hydrolysis, and beta-galactosidase. Among a total of 359 strains tested, eight biotypes were observed, the most common ones being types 1 (beta-galactosidase positive, lipase positive, hippurate positive), 2 (beta-galactosidase negative, lipase positive...

Humoral circulatory immune response to Gardnerella vaginalis.

Strain-specific circulating immunoglobulin G and/or M was detected by enzyme-linked immunosorbent assay and immunofluorescence test by using Formol-treated suspensions of Gardnerella vaginalis from 28 women with overt vaginitis but only three symptom-free subjects among 43 otherwise healthy women found to be colonized by G. vaginalis. Analogous but less stringent strain specificity patterns wer...

[Sexual transmission of Gardnerella vaginalis].

Male carriage of Gardnerella vaginalis.

The prevalence of Gardnerella vaginalis in the urethra of 430 men attending a clinic for sexually transmitted disease was 11·4%; it was significantly higher in heterosexuals (14·5%) than in homosexuals (4·5%). There was no evidence of rectal or subpreputial carriage of G vaginalis, and urethral carriage was not associated with symptoms of urethritis.